| FT3

Free triiodothyronine (FT3) is a hormone synthesized and secreted by thyroid follicular cells. It constitutes about 0.3% of T3 and can cross cell membranes to enter tissues and exert physiological effects. Its concentration aligns with the tissue level of triiodothyronine and corresponds to the body's metabolic state. FT3 also holds diagnostic value for non-thyroid diseases.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	0.216	0.298	0.417	0.669	0.955	1.405	1.79	1.865

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of FT3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	80-105	97
Plasma(n=8)	93-108	102
Cell Culture Supernates(n=8)	95-107	103

 

| Linearity

Samples were spiked with high concentrations of FT3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	78-97	89-100	91-112
	Average (%)	94	93	97
1 : 4	Range (%)	90-109	95-109	101-116
	Average (%)	93	98	111